| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 09 Boys |
0443-01SLB3U-0071 | Davis Draper |
| A2 | Blount United Soccer Club Blount United Soccer Club - B13U |
0201-012B3U-0231 | Darren Sutton |
| A3 | Chattanooga FC Academy Chattanooga FC Academy - 2009B Navy |
0316-022B3U-7826 | Wesley Ford |
| A4 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 09B Red |
0324-022B3U-0031 | Josue Fernandez |
| A5 | Emerald Force Soccer Club - 01 Emerald Force 09 Manchester City |
0214-012B3U-1246 | Kelvin Scott |
| A6 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B3U-0004 | Izudin Operta |
| A7 | Kings Hammer MSC 09 Strikers Showcase |
0515-012B3U-0122 | Fifi Dadson |
| A9 | Wilson United Soccer Club Wilson United - B13U |
0406-012B3U-0006 | Philip Hayes |