| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2009B White |
0316-022B3U-7827 | Thomas Bentham |
| A2 | Knoxville FC (Crush) Knoxville FC Crush 09 B Premier |
0206-012B3U-0130 | Jorge Valdivia |
| A3 | Knoxville Reds Football Club - 01 Knoxville Reds 09 Juventus |
0290-01D3B3U-0009 | Mark Johnson |
| A4 | Kings Hammer MSC 09 Strikers Premier |
0515-013B3U-0123 | Paul Parkerson |
| A5 | Soccer Club of Oak Ridge - 01 SCOR 09 BU13 |
0208-013B3U-0006 | B Moua - DO NOT USE |